Environmental testing of the Orion European Structural Test Article (E-STA), which contains the Orion European Service Module (ESM), required that the onboard propellant tanks be filled and drained with fuel and oxidizer simulant fluids as well as pressurized and de-pressurized with an ullage gas. This conference paper will elaborate on how these objectives were fulfilled by presenting the development of derived requirements definition, initial fill and drain concepts, selection of simulant fluids, finalization of pump and pressurization design, selection of components, selection of transfer hoses and interface connections, as well as development and maintenance of budgets, schedules, reviews, construction, documentation, and test procedures. This paper also describes implementation of checkout and commissioning activities leading to successful fluid cart pumping and pressurization operations for the test campaign. The development, construction, and operation of the fluid cart pumping and pressurization systems for the Environmental testing of the Orion European Structural Test Article (E-STA), took place at the NASA Glenn Research Center's Plum Brook Station Space Environments Complex (SEC) during 2015 and 2016.