The mission of the National Aeronautics and Space Administration is to reach for new heights for the benefit of humankind. In order for space flight to be feasible over further distances and longer durations of time, food must be sustainably produced under the unique conditions of space. Freeze drying, the method commonly used by astronauts for food supply, is not suitable for prolonged space flight because the food loses its nutritional value as time passes. Kennedy Space Center’s Utilization and Life Sciences Office (UB-A), under the Exploration Research and Technology Program (UB), conducts research on plant growth and development under International Space Station (ISS) conditions. The New Crop Selection experiments are testing the suitability of leafy crops to ISS conditions. Through this particular experiment, we seek to optimize food production by analyzing the growth responses of leafy greens through the manipulation of CO2 levels.