In 2017, the combined Optical Telescope Element and Integrated Science Instrument Module (OTIS) of the James Webb Space Telescope (JWST) underwent functional testing and optical metrology verification under cryogenic vacuum conditions in Chamber A at the Johnson Space Center (JSC). Testing the infrared Science Instruments and OTIS optics below 50 degrees Kelvin required an environment architecture that comprehensively controlled the temperature and energy path of every seam and penetration in the over 1100 m2 of Chamber A helium shroud surfaces as well as the Ground Support Equipment (GSE) inside it. This paper outlines the design and implementation of thermal closeouts, thermal anchoring systems for electrical cables, and thermal control systems around room-temperature optical metrology equipment inside the helium shroud. It also details lessons learned from the repeated implementation and testing of these environmental control systems throughout the JWST Pathfinder test campaign.