This paper offers a comprehensive view of flight system integration and test (I&T) lessons learned related to mishaps and close calls, focusing on what can be done to improve the I&T process to avoid recurrence. Specific areas within the realm of I&T that are covered in this paper include: I&T team communication and training; design of flight and ground systems for I&T; planning and scheduling; configuration management and process documentation; ground support equipment and tools; cleanrooms and contamination; mechanical integration, handling, and deployments; electrical integration and electrostatic discharge; functional testing and troubleshooting; environmental testing and facilities; and launch site operations. To illustrate "real-world" lessons learned for I&T, examples from throughout the history of the U.S. space program are presented. Also presented are some best practices for I&T that can help mitigate mishaps and close calls on the ground or during flight.