Conservation of Modules but not Phenotype in Bacterial Response to Environmental Stress

Timberlake, Sonia ; Joachimiak, Marcin ; Joyner, Dominique ; Chakraborty, Romy ; Baumohl, Jason ; Dehal, Paramvir ; Arkin, Adam ; Hazen, Terry ; Alm, Eric

Lawrence Berkeley National Laboratory

关键词: Gene Regulation;    Genes;    54;    Regulations Transcriptional Response, General Stress Response, Transcriptional Modules;    Phenotype;   

DOI  :  10.2172/985921
RP-ID  :  LBNL-3744E-Poster
RP-ID  :  DE-AC02-05CH11231
RP-ID  :  985921

美国|英语

来源: UNT Digital Library

【 摘 要 】

Microbes live in changing environments and change their phenotype via gene regulation in response. Although this transcriptional response is important for fitness, very little is known about how it evolves in microbes. We started by asking a number of high-level questions about the evolution of transcriptional phenotype: (1) To what extent is transcriptional response conserved, i.e. do conserved genes respond similarly to the same condition; (2) To what extent are transcriptional modules conserved; and (3) Does there exist a general stress response to a variety of stressors? To illuminate these questions, we analyzed more than 500 microarray experiments across the bacterial domain. We looked for conservation of transcriptional regulation both in close sister species and vastly divergent clades. In addition, we produced and analyzed an extensive in-house compendium of environmental stress data in three metal-reducing bacteria.