Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris

Rajeev, Lara ; Luning, Eric ; Dehal, Paramvir ; Joachimiak, Marcin ; Mukhopadhyay, Aindrila

Lawrence Berkeley National Laboratory

关键词: Dna;    58;    D. Vulgaris Hildenborough, Sulfate-Reducing Bacteria, Dna-Affinity-Purification-Chip (Dap-Chip);    54;    Promoters;   

DOI  :  10.2172/985735
RP-ID  :  LBNL-3687E-Poster
RP-ID  :  DE-AC02-05CH11231
RP-ID  :  985735

美国|英语

来源: UNT Digital Library

【 摘 要 】

D. vulgaris Hildenborough has 72 response regulators. The Desulfovibrio are sulfate reducing bacteria that are important in the sulfur and carbon cycles in anoxic habitats. Its large number of two componenent systems are probably critical to its ability to sense and respond to its environment. Our goal is to map these RRs to the genes they regulate using a DNA-affinity-purification-chip (DAP-chip) protocol. First target determined usuing EMSA. A positive target was determined for as many RRs as possible using EMSA. Targets were selected based on gene proximity, regulon predictions and/or predicted sigma54 dependent promoters. qPCR was used to ensure that the target was enriched from sheared genomic DNA before proceeding to the DAP-chip.