Preliminary performance assessment of biotoxin detection for UWS applications using a MicroChemLab device.

VanderNoot, Victoria A. ; Haroldsen, Brent L. ; Renzi, Ronald F. ; Shokair, Isaac R.

Sandia National Laboratories

关键词: Water Chemistry.;    Biological Warfare Agents;    Toxins;    Monitoring;    Biological Weapons.;   

DOI  :  10.2172/984154
RP-ID  :  SAND2010-1147
RP-ID  :  AC04-94AL85000
RP-ID  :  984154

美国|英语

来源: UNT Digital Library

【 摘 要 】

In a multiyear research agreement with Tenix Investments Pty. Ltd., Sandia has been developing field deployable technologies for detection of biotoxins in water supply systems. The unattended water sensor or UWS employs microfluidic chip based gel electrophoresis for monitoring biological analytes in a small integrated sensor platform. This instrument collects, prepares, and analyzes water samples in an automated manner. Sample analysis is done using the {mu}ChemLab{trademark} analysis module. This report uses analysis results of two datasets collected using the UWS to estimate performance of the device. The first dataset is made up of samples containing ricin at varying concentrations and is used for assessing instrument response and detection probability. The second dataset is comprised of analyses of water samples collected at a water utility which are used to assess the false positive probability. The analyses of the two sets are used to estimate the Receiver Operating Characteristic or ROC curves for the device at one set of operational and detection algorithm parameters. For these parameters and based on a statistical estimate, the ricin probability of detection is about 0.9 at a concentration of 5 nM for a false positive probability of 1 x 10{sup -6}.