Feasibility of Single Molecule DNA Sequencing using Surface-Enhanced Raman Scattering

Talley, C E ; Reboredo, F ; Chan, J ; Lane, S M

Lawrence Livermore National Laboratory

关键词: 75 Condensed Matter Physics, Superconductivity And Superfluidity;    37 Inorganic, Organic, Physical And Analytical Chemistry;    59 Basic Biological Sciences;    Shape;    Dna Sequencing;   

DOI  :  10.2172/899105
RP-ID  :  UCRL-TR-218737
RP-ID  :  W-7405-ENG-48
RP-ID  :  899105

美国|英语

来源: UNT Digital Library

【 摘 要 】

We have used a combined theoretical and experimental approach in order to assess the feasibility of using surface-enhanced Raman scattering (SERS) for DNA sequencing at the single molecule level. We have developed a numerical tool capable of calculating the E-field and resulting SERS enhancement factors for metallic structures of arbitrary size and shape. Measurements of the additional SERS enhancement by combining SERS with coherent antistokes Raman scattering (CARS) show that only modest increases in the signal are achievable due to thermal damage at higher laser powers. Finally, measurements of the SERS enhancement from nanoparticles coated with an insulating layer show that the SERS enhancement is decreased by as much as two orders of magnitude when the molecule is not in contact with the metal surface.