Molecular Mechanism of Uranium Reduction by Clostridia and its Manipulation

Francis, A. J. ; W. Gao, D. Chidambaram ; Dodge, C.J.

Brookhaven National Laboratory

关键词: Uranium;    Microorganisms;    Hydrogenases;    Enzymes;    Biochemistry;   

DOI  :  10.2172/896246
RP-ID  :  ERSD-1026860-2006
RP-ID  :  None
RP-ID  :  896246

美国|英语

来源: UNT Digital Library

【 摘 要 】

This research addresses the need for detailed studies of the enzymatic mechanisms for reduction of radionuclides and/or metals by fermentative microorganisms. The overall objective of this research is to elucidate systematically the molecular mechanisms involved in the reduction of uranium by Clostridia. We propose to (1) determine the role of hydrogenases in uranium reduction, (22) purify the enzymes involved in uranium reduction, (3) determine the mechanisms of reduction, e.g., one or two electron transfer reactions, and (4) elucidate the genetic control of the enzymes and cellular factors involved in uranium reduction. This is a collaborative study between BNL and Stanford University involving expertise in biomolecular science, biochemistry, microbiology, and electrochemistry.