Promoting uranium immobilization by the activities of microbial phophatases

Sobecky, Patricia A.

Georgia Institute of Technology

关键词: Alkaline Phosphatase;    Uranium;    Microorganisms;    Genes;    37 Inorganic, Organic, Physical And Analytical Chemistry;   

DOI  :  10.2172/893417
RP-ID  :  NABIR-1024775-2005
RP-ID  :  None
RP-ID  :  893417

美国|英语

来源: UNT Digital Library

【 摘 要 】

The first objective of this project is to determine the relationship of phosphatase activity to metal resistance in subsurface strains and the role of lateral gene transfer (LGT) in dissemination of nonspecific acid phosphatase genes. Nonspecific acid phosphohydrolases are a broad group of secreted microbial phosphatases that function in acidic-to-neutral pH ranges and utilize a wide range of organophosphate substrates. We have previously shown that PO43- accumulation during growth on a model organophosphorus compound was attributable to the overproduction of alkaline phosphatase by genetically modified subsurface pseudomonads [Powers et al. (2002) FEMS Microbiol. Ecol. 41:115-123]. During this report period, we have extended these results to include indigenous metal resistant subsurface microorganisms cultivated from the Field Research Center (FRC), in Oak Ridge Tennessee.