Final Technical Report

Yanch, Jacquelyn

Massachusetts Institute of Technology

关键词: Boron 10;    Computerized Tomography;    Neutron Activation Analysis;    Rheumatic Diseases;    Computerized Simulation;   

DOI  :  10.2172/882513
RP-ID  :  DOE-14343-04
RP-ID  :  fg07-02ID14343
RP-ID  :  882513

美国|英语

来源: UNT Digital Library

【 摘 要 】

This project involved the development of a method for in vivo prompt gamma neutron activation analysis for the investigation of Boron-10 distribution in a rabbit knee. The overall objective of this work was a robust approach for rapid screening of new {sup 10}B-labelled compounds to determine their suitability for use in the treatment of rheumatoid arthritis via Boron Neutron Capture Synovectomy (BNCS). For BNCS it is essential to obtain a compound showing high uptake levels in the synovium and long residence time in the joints. Previously the in vivo uptake behavior of potential compounds was evaluated in the arthritic knee joints of rabbits via extensive dissection studies. These studies are very labor-intensive and involve sacrificing large numbers of animals. An in vivo {sup 10}B screening approach was developed to provide initial evaluation of potential compounds. Only those compounds showing positive uptake and retention characteristics will be evaluated further via dissection studies. No further studies will be performed with compounds showing rapid clearance and/or low synovial uptake. Two approaches to in vivo screening were investigated using both simulation methods and experimentation. Both make use of neutron beams generated at the MIT Research Reactor. The first, Transmission Computed Tomography (TCT) was developed and tested but was eventually rejected due to very limited spatial resolution using existing reactor beams. The second, in vivo prompt gamma neutron activation analysis (IVPGNAA) was much more promising. IVPGNAA was developed using computer simulation and physical measurement coupled with image reconstruction techniques. The method was tested in arthritic New Zealand rabbits previously injected intra-articularly with three boron labeled compounds and shown to be effective in providing information regarding uptake level and residence time of {sup 10}B in the joint.