IDENTIFICATION AND CHARACTERIZATION OF THERMOBIFIDA FUSCA GENES INVOLVED IN PLANT CELL WALL DEGRADATION.

Wilson, David B.

Cornell University

关键词: Biomass;    Crystallography;    Cellulose;    Plant Cells;    59 Basic Biological Sciences;   

DOI  :  10.2172/862421
RP-ID  :  Final Technical Report
RP-ID  :  FG02-01ER63150
RP-ID  :  862421

美国|英语

来源: UNT Digital Library

【 摘 要 】

Micro-array experiments identified a number of Thermobifida fusca genes which were upregulated by growth on cellulose or plant biomass. Five of these genes were cloned, overexpressed in E. coli and the expressed proteins were purified and characterized. These were a xyloglucanase,a 1-3,beta glucanase, a family 18 hydrolase and twocellulose binding proteins that contained no catalytic domains. The catalyic domain of the family 74 endoxyloglucanase with a C-terminal, cellulose binding module was crystalized and its 3-dimensional structure was determined by X-ray crystallography.