Final Report on Grant DE-FG-02ER63350

Miller, John H.

Washington State University

关键词: Removal;    Guanine;    Dna;    Enzymes;    Oxidation;   

DOI  :  10.2172/840787
RP-ID  :  DOE/ER/63350-1
RP-ID  :  FG03-02ER63350
RP-ID  :  840787

美国|英语

来源: UNT Digital Library

【 摘 要 】

Research funded by grant DE-FG-02ER63350 focused on DNA bending measured NMR spectroscopy and modeled by classical molecular dynamics (MD) simulation. Bending is a structural aspect of DNA that is plays a key role in its function. The most important finding of our research was that oxidation of guanine, a ubiquitous DNA lesion caused by endogenous and environmental oxidative stress, changes DNA bending dynamics in a way that favors binding of glycosylases, repair enzymes that remove damaged bases from DNA. Hence, the effect of 8-oxoguanine on DNA bending contributes to its recognition and removal by the base excision repair system.