Engineered Antibodies for Monitoring of Polynuclear Aromatic Hydrocarbons

Karu, Alexander E. ; Roberts, Victoria A. ; Li, Qing X.

University of California, Berkeley

关键词: Antibodies;    Decontamination;    Polycyclic Aromatic Hydrocarbons;    37 Inorganic, Organic, Physical And Analytical Chemistry;    Genetic Engineering;   

DOI  :  10.2172/825746
RP-ID  :  EMSP-54546--2000
RP-ID  :  FG07-96ER62316
RP-ID  :  825746

美国|英语

来源: UNT Digital Library

【 摘 要 】

Polynuclear aromatic hydrocarbons (PAHs) are a large class of structurally similar pollutants. Rapid, inexpensive, and high-throughput methods to identify and monitor PAHs are needed in several DOE focus areas, including human and ecosystem health effects, risk and exposure assessment, decontamination and decommissioning, and remediation. DOE has sponsored and participated in several demonstration projects in which commercial immunoassay kits proved useful and cost-effective for detection of PAHs and other pollutants. The emerging generation of sensors and residue recovery methods will require panels of antibodies with relatively subtle differences in cross-reactivity. This project is based on the premise that genetic engineering should be much more successful than conventional polyclonal and monoclonal antibody methods for developing these antibody panels. One objective of this project has been to define the structural basis and mechanisms by which antibodies bind and cross-react with various PAHs. A second objective has been to use this information to produce recombinant antibodies with improved performance in analytical procedures that DOE can use. A third objective has been development of PAH residue recovery and cleanup methods that will be compatible with immunoassays, and make instrumental analysis faster, more accurate, and less expensive.