DOE Final report [A genetic analysis of the lumenal proteins of the PSII O{sub 2} evolving complex of cyanobacteria]

Sherman, Louis A.

Purdue University, West Lafayette, IN (United States)

关键词: Genetics;    Oxygen;    Genes;    59 Basic Biological Sciences;    Proteins;   

DOI  :  10.2172/811277
RP-ID  :  DOE/ER/14028--1
RP-ID  :  FG02-89ER14028
RP-ID  :  811277

美国|英语

来源: UNT Digital Library

【 摘 要 】

The primary objectives of this proposal were a better understanding of the structure and function of the Mn-stabilizing (MSP) in two cyanobacteria, Synechocystis sp. PCC6803 and Cyanothece sp. ATCC51142. In addition, the author was interested in analysis of the other cyanobacteria lumenal PSII proteins, cyt c{sub 550} and the 12 kDa protein. The experimentation involved analysis of targeted random mutagenesis of the genes encoding the three proteins, especially in psbO, the gene encoding MSP. This required mutant screening with a digital imaging spectrometer. Knockout mutants of the other two lumenal proteins in Synechocystis sp. PCC6803 were also constructed. In addition, photosynthetic O{sub 2} evolution in Cyanothece sp. ATCC51142, a nitrogen-fixing strain that regulates the activity of nitrogenase and photosynthesis in a temporal fashion, was analyzed.