Distributed Sensor Particles for Remote Fluorescence Detection of Trace Analytes: UXO/CW

SINGH, ANUP K. ; GUPTA, ALOK ; MULCHANDANI, ASHOK ; CHEN, WILFRED ; BHATIA, RIMPLE B. ; SCHOENIGER, JOSEPH S. ; ASHLEY, CAROL S. ; BRINKER, C. JEFFREY ; HANCE, BRADLEY G. ; SCHMITT, RANDAL L. ; JOHNSON, MARK S. ; HARGIS JR., PHILIP J. ; SIMONSON, ROBERT J.

Sandia National Laboratories

关键词: Amplification;    Sand;    Tnt;    Fluorescence;    Polymers;   

DOI  :  10.2172/789593
RP-ID  :  SAND2001-3522
RP-ID  :  AC04-94AL85000
RP-ID  :  789593

美国|英语

来源: UNT Digital Library

【 摘 要 】

This report summarizes the development of sensor particles for remote detection of trace chemical analytes over broad areas, e.g residual trinitrotoluene from buried landmines or other unexploded ordnance (UXO). We also describe the potential of the sensor particle approach for the detection of chemical warfare (CW) agents. The primary goal of this work has been the development of sensor particles that incorporate sample preconcentration, analyte molecular recognition, chemical signal amplification, and fluorescence signal transduction within a ''grain of sand''. Two approaches for particle-based chemical-to-fluorescence signal transduction are described: (1) enzyme-amplified immunoassays using biocompatible inorganic encapsulants, and (2) oxidative quenching of a unique fluorescent polymer by TNT.