Rapid Generation of a Nanocrystal-Labeled Peptide Library for Specific Identification of the Bacterium Clostrium Botulinum

Tok, J B

Lawrence Livermore National Laboratory

关键词: Amino Acids;    Kinetics;    Tetanus;    37 Inorganic, Organic, Physical And Analytical Chemistry;    Plasmons;   

DOI  :  10.2172/15011397
RP-ID  :  UCRL-TR-208082
RP-ID  :  W-7405-ENG-48
RP-ID  :  15011397

美国|英语

来源: UNT Digital Library

【 摘 要 】

Several peptide libraries containing up to 2 million unique peptide ligands have been synthesized. The peptides are attached onto a 80 micron resin and the length of these peptide ligands ranges from 5 to 9 amino acid residues. Using a novel calorimetric assay, the libraries were screened for binding to the ganglioside-binding domain of Clostridium Tetanus Toxin, a structural similar analog of the Clostridium Botulinum toxin. Several binding peptide sequences were identified, in which the detailed binding kinetics are currently underway using the Surface Plasmon Resonance (SPR) technique.