科技报告详细信息
Rapid Generation of a Nanocrystal-Labeled Peptide Library for Specific Identification of the Bacterium Clostrium Botulinum | |
Tok, J B | |
Lawrence Livermore National Laboratory | |
关键词: Amino Acids; Kinetics; Tetanus; 37 Inorganic, Organic, Physical And Analytical Chemistry; Plasmons; | |
DOI : 10.2172/15011397 RP-ID : UCRL-TR-208082 RP-ID : W-7405-ENG-48 RP-ID : 15011397 |
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美国|英语 | |
来源: UNT Digital Library | |
【 摘 要 】
Several peptide libraries containing up to 2 million unique peptide ligands have been synthesized. The peptides are attached onto a 80 micron resin and the length of these peptide ligands ranges from 5 to 9 amino acid residues. Using a novel calorimetric assay, the libraries were screened for binding to the ganglioside-binding domain of Clostridium Tetanus Toxin, a structural similar analog of the Clostridium Botulinum toxin. Several binding peptide sequences were identified, in which the detailed binding kinetics are currently underway using the Surface Plasmon Resonance (SPR) technique.
【 预 览 】
Files | Size | Format | View |
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15011397.pdf | 1385KB | download |