Rapid Detection of Biological and Chemical Threat Agents Using Physical Chemistry, Active Detection, and Computational Analysis

Chung, Myung ; Dong, Li ; Fu, Rong ; Liotta, Lance ; Narayanan, Aarthi ; Petricoin, Emanuel ; Ross, Mark ; Russo, Paul ; Zhou, Weidong ; Luchini, Alessandra ; Manes, Nathan ; Chertow, Jessica ; Han, Suhua ; Kidd, Jessica ; Senina, Svetlana ; Groves, Stephanie

National Center for Biodefense and Infectious Diseases, George Mason University. Fairfax VA

关键词: 45 Military Technology, Weaponry, And National Defense;   

DOI  :  10.2172/1049503
RP-ID  :  DOE/NA/25455
RP-ID  :  FC52-04NA25455
RP-ID  :  1049503

美国|英语

来源: UNT Digital Library

【 摘 要 】

Basic technologies have been successfully developed within this project: rapid collection of aerosols and a rapid ultra-sensitive immunoassay technique. Water-soluble, humidity-resistant polyacrylamide nano-filters were shown to (1) capture aerosol particles as small as 20 nm, (2) work in humid air and (3) completely liberate their captured particles in an aqueous solution compatible with the immunoassay technique. The immunoassay technology developed within this project combines electrophoretic capture with magnetic bead detection. It allows detection of as few as 150-600 analyte molecules or viruses in only three minutes, something no other known method can duplicate. The technology can be used in a variety of applications where speed of analysis and/or extremely low detection limits are of great importance: in rapid analysis of donor blood for hepatitis, HIV and other blood-borne infections in emergency blood transfusions, in trace analysis of pollutants, or in search of biomarkers in biological fluids. Combined in a single device, the water-soluble filter and ultra-sensitive immunoassay technique may solve the problem of early “warning type” detection of aerosolized pathogens. These two technologies are protected with five patent applications and are ready for commercialization.