Interim report on updated microarray probes for the LLNL Burkholderia pseudomallei SNP array

Gardner, S ; Jaing, C

Lawrence Livermore National Laboratory

关键词: Lawrence Livermore National Laboratory;    Genetics;    59 Basic Biological Sciences;    Design;    60 Applied Life Sciences;   

DOI  :  10.2172/1047245
RP-ID  :  LLNL-TR-543366
RP-ID  :  W-7405-ENG-48
RP-ID  :  1047245

美国|英语

来源: UNT Digital Library

【 摘 要 】

The overall goal of this project is to forensically characterize 100 unknown Burkholderia isolates in the US-Australia collaboration. We will identify genome-wide single nucleotide polymorphisms (SNPs) from B. pseudomallei and near neighbor species including B. mallei, B. thailandensis and B. oklahomensis. We will design microarray probes to detect these SNP markers and analyze 100 Burkholderia genomic DNAs extracted from environmental, clinical and near neighbor isolates from Australian collaborators on the Burkholderia SNP microarray. We will analyze the microarray genotyping results to characterize the genetic diversity of these new isolates and triage the samples for whole genome sequencing. In this interim report, we described the SNP analysis and the microarray probe design for the Burkholderia SNP microarray.