Determining mutant spectra of three RNA viral samples using ultra-deep sequencing

Chen, H

Lawrence Livermore National Laboratory

关键词: Viruses;    Spectra;    Animals;    59 Basic Biological Sciences;    Rna;   

DOI  :  10.2172/1044235
RP-ID  :  LLNL-TR-560431
RP-ID  :  W-7405-ENG-48
RP-ID  :  1044235

美国|英语

来源: UNT Digital Library

【 摘 要 】

RNA viruses have extremely high mutation rates that enable the virus to adapt to new host environments and even jump from one species to another. As part of a viral transmission study, three viral samples collected from naturally infected animals were sequenced using Illumina paired-end technology at ultra-deep coverage. In order to determine the mutant spectra within the viral quasispecies, it is critical to understand the sequencing error rates and control for false positive calls of viral variants (point mutantations). I will estimate the sequencing error rate from two control sequences and characterize the mutant spectra in the natural samples with this error rate.