Improving public health emergency preparedness is at the top of the national agenda, but the lack of frequent opportunities to observe and learn from real-world responses to large-scale public health emergencies has hindered the development of an adequate evidence base. As a result, efforts to develop performance measures and standards, best practices, program guidance, training, and other tools have proceeded without a strong empirical and analytical basis. The Office of the Assistant Secretary for Preparedness and Response asked RAND to facilitate the development of a public health systems research agenda for emergency preparedness, identify short- and long-term research priorities, and provide a basis for coordinating funders and researchers inside and outside the federal government. In response, RAND convened a panel of 13 experts representing a diverse range of perspectives. The panelists identified 20 research priorities and illustrative research questions in areas related to planning, response, resources and infrastructure, and accountability and improvement. Preparations for the panel discussion included an extensive literature review of peer-reviewed sources and statutes, policies, and other government and organizational reports.