Analysis of Hexanitrostilbene (HNS) and Dipicryethane (DPE) for Mutagenicity by the Ames/Salmonella Assay

Wu, R ; Felton, J

关键词: BACTERIA;    ENZYMES;    GENES;    HISTIDINE;    HOMOGENATES;    LIVER;    METABOLIC ACTIVATION;    MUTATIONS;    POPULATIONS;    SALMONELLA;    SALMONELLA TYPHIMURIUM;    STRAINS;   

DOI  :  10.2172/923094
RP-ID  :  UCRL-TR-235652
PID  :  OSTI ID: 923094
Others  :  TRN: US200806%%357

美国|英语

来源: SciTech Connect

【 摘 要 】

The Ames/Salmonella assay, developed by Professor Bruce Ames at the University of California, Berkeley, is a rapid and sensitive assay for detecting mutagenicity of various chemical compounds (Maron and Ames, 1983). It is a widely accepted short-term assay for detecting chemicals that induce mutations in the histidine (his) gene of Salmonella typhimurium. This is a reverse mutation assay that detects the mutational reversion of his-dependent Salmonella to the his-independent counterpart. Thereby, mutagenic compounds will increase the frequency of occurrence of his-independent bacterial colonies. The assay utilizes the specific genetically constructed strains of bacteria either with or without mammalian metabolic activation enzymes (S9), Aroclor induced rat liver homogenate to assess the mutagenicity of different compounds. In this study, we will use the Ames/Salmonella assay to investigate the mutagenicity of Hexanitrostilbene (HNS) from both Bofors and Pantex, and Dipicryethane (DPE).