Si Nanopores Development for External Control of Transport of Biomolecules

Ileri, N ; Tringe, J ; Letant, S ; Palozoglu, A ; Stroeve, P ; Faller, R

关键词: BIOTECHNOLOGY;    EFFICIENCY;    FABRICATION;    GREECE;    HYPOTHESIS;    MEMBRANES;    MOLECULAR SIEVES;    POLYCARBONATES;    PROTEINS;    SILICON;    SYNTHESIS;    TRAINING;    TRANSPORT;    VIRUSES;   

DOI  :  10.2172/945548
RP-ID  :  LLNL-TR-404916
PID  :  OSTI ID: 945548
Others  :  TRN: US0901211

美国|英语

来源: SciTech Connect

【 摘 要 】

Nazar Ileri has been involved in an independent, multidisciplinary effort to create a new class of molecular sieves for proteins and viruses. Her experimental work has been performed concurrently at two campuses, LLNL and UC Davis, while theoretical components have been largely accomplished at UC Davis. As will be described, the devices she is creating have great potential to improve very significantly the efficiency and selectivity of molecular transport over what is presently available from state-of-the-art membranes. Our biotechnology training program is based on an integrated study of the transport of biomolecules through conically-shaped, nanoporous silicon membranes. The overall objective of this effort is to demonstrate an efficient, highly selective membrane technology that is manufacturable for macroscopic areas and can be employed in sensing, diagnostic and biomedical applications. Our specific aims are to (1) fabricate and characterize the physical characteristics of the membranes, (2) to demonstrate their utility for molecular transport and separation, and (3) to develop models that will facilitate understanding of these devices as well as improved performance of the next generation of devices. We have proposed that the conical pores have superior performance characteristics compared to other porous filters. To study this hypothesis, complementary approaches from different disciplines, such as membrane synthesis, experiment, and molecular simulation need to be combined. This provides an ideal training environment for a future leader in biotechnology. Hence, for this study, Nazar Ileri has started to carry out a full range of experimental and theoretical investigations under our guidance. First, she has begun fabrication of filters with conical/pyramidal pores. She characterized the pores by AFM and SEM, and analyzed the images using wavelets and other mathematical tools. She has also started to conduct biomolecule transport experiments to compare the efficiency of fabricated filters vs. state-of-the-art commercial polycarbonate track-etched (PCTE) membranes. Finally, she has performed preliminary molecular calculations to investigate the operating principles of such systems and she has obtained results which she will present at the international 'Nanostructured materials, membrane modeling and simulation' workshop in Greece.