Final Technical Report

Michael Laub

关键词: BACTERIA;    BIOLOGY;    CELL CYCLE;    GLOBAL ANALYSIS;    HISTIDINE;    METABOLISM;    PHOSPHOTRANSFERASES;    PROTEINS;   

DOI  :  10.2172/944810
RP-ID  :  DOE-ER04-07-4 Final Report
PID  :  OSTI ID: 944810
Others  :  TRN: US201003%%187

学科分类：生物科学（综合）

美国|英语

来源: SciTech Connect

【 摘 要 】

Our team of investigators from MIT (Michael Laub) and Stanford (Harley McAdams and Lucy Shapiro) conducted a multi-faceted, systematic experimental analysis of the 106 Caulobacter two-component signal transduction system proteins (62 histidine kinases and 44 response regulators) to understand how they coordinate cell cycle progression, metabolism, and response to environmental changes. These two-component signaling proteins were characterized at the genetic, biochemical, and genomic levels. The results generated by our laboratories have provided numerous insights into how Caulobacter cells sense and respond to a myriad of signals. As nearly all bacteria use two-component signaling for cell regulation, the results from this project help to deepen our general understanding of bacterial signal transduction. The tools and approaches developed can be applied to other bacteria. In particular, work from the Laub laboratory now enables the systematic, rational rewiring of two-component signaling proteins, a major advance that stands to impact synthetic biology and the development of biosensors and other designer molecular circuits. Results are summarized from our work. Each section lists publications and publicly-available resources which result from the work described.