Shotgun protein sequencing.

Faulon, Jean-Loup Michel ; Heffelfinger, Grant S.

关键词: AMINO ACID SEQUENCE;    DIGESTION;    DNA;    MIXTURES;    MODIFICATIONS;    PEPTIDES;    PROTEINS;   

DOI  :  10.2172/959081
RP-ID  :  SAND2009-3855
PID  :  OSTI ID: 959081
Others  :  TRN: US201016%%549

美国|英语

来源: SciTech Connect

【 摘 要 】

A novel experimental and computational technique based on multiple enzymatic digestion of a protein or protein mixture that reconstructs protein sequences from sequences of overlapping peptides is described in this SAND report. This approach, analogous to shotgun sequencing of DNA, is to be used to sequence alternative spliced proteins, to identify post-translational modifications, and to sequence genetically engineered proteins.