Producing Conditional Mutants for Studying Plant Microtubule Function

Richard Cyr

关键词: BIOSPHERE;    CELL CYCLE;    CELLULOSE;    DEPOSITION;    GENES;    MICROTUBULES;    MUTANTS;    POLYMERS;   

DOI  :  10.2172/964930
RP-ID  :  DOE91ER20050- Final Report
PID  :  OSTI ID: 964930
Others  :  TRN: US201110%%440

学科分类：生物科学（综合）

美国|英语

来源: SciTech Connect

【 摘 要 】

The cytoskeleton, and in particular its microtubule component, participates in several processes that directly affect growth and development in higher plants. Normal cytoskeletal function requires the precise and orderly arrangement of microtubules into several cell cycle and developmentally specific arrays. One of these, the cortical array, is notable for its role in directing the deposition of cellulose (the most prominent polymer in the biosphere). An understanding of how these arrays form, and the molecular interactions that contribute to their function, is incomplete. To gain a better understanding of how microtubules work, we have been working to characterize mutants in critical cytoskeletal genes. This characterization is being carried out at the subcellular level using vital microtubule gene constructs. In the last year of funding colleagues have discovered that gamma-tubulin complexes form along the lengths of cortical microtubules where they act to spawn new microtubules at a characteristic 40 deg angle. This finding complements nicely the finding from our lab (which was funded by the DOE) showing that microtubule encounters are angle dependent; high angles encounters results in catastrophic collisions while low angle encounters result in favorable zippering. The finding of a 40 deg spawn of new microtubules from extant microtubule, together with aforementioned rules of encounters, insures favorable co-alignment in the array. I was invited to write a New and Views essay on this topic and a PDF is attached (News and Views policy does not permit funding acknowledgments and so I was not allowed to acknowledge support from the DOE).