Single cell analysis: the new frontier in 'Omics'

Wang, Daojing ; Bodovitz, Steven

关键词: 59;    BIOLOGY;    DNA SEQUENCERS;    GENES;    MASS SPECTROSCOPY;    METABOLITES;    PROTEINS;   

DOI  :  10.2172/983315
RP-ID  :  LBNL-3480E
PID  :  OSTI ID: 983315
Others  :  TRN: US201014%%605

美国|英语

来源: SciTech Connect

【 摘 要 】

Cellular heterogeneity arising from stochastic expression of genes, proteins, and metabolites is a fundamental principle of cell biology, but single cell analysis has been beyond the capabilities of 'Omics' technologies. This is rapidly changing with the recent examples of single cell genomics, transcriptomics, proteomics, and metabolomics. The rate of change is expected to accelerate owing to emerging technologies that range from micro/nanofluidics to microfabricated interfaces for mass spectrometry to third- and fourth-generation automated DNA sequencers. As described in this review, single cell analysis is the new frontier in Omics, and single cell Omics has the potential to transform systems biology through new discoveries derived from cellular heterogeneity.