Improvements In Ethanologenic Escherichia Coli and Klebsiella Oxytoca | |
Dr. David Nunn | |
关键词: BIOMASS; DISTILLATION; ENZYMES; ESCHERICHIA COLI; ETHANOL; FERMENTATION; HEXOSES; KLEBSIELLA; PENTOSES; SACCHARIDES; SACCHARIFICATION; STRAINS; TOLERANCE; | |
DOI : 10.2172/992134 RP-ID : DOEGO17058 PID : OSTI ID: 992134 Others : TRN: US201022%%387 |
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学科分类:燃料技术 | |
美国|英语 | |
来源: SciTech Connect | |
【 摘 要 】
The current Verenium cellulosic ethanol process is based on the dilute-acid pretreatment of a biomass feedstock, followed by a two-stage fermentation of the pentose sugar-containing hydrolysate by a genetically modified ethanologenic Escherichia coli strain and a separate simultaneous saccharification-fermentation (SSF) of the cellulosic fraction by a genetically modified ethanologenic Klebsiella oxytoca strain and a fungal enzyme cocktail. In order to reduce unit operations and produce a fermentation beer with higher ethanol concentrations to reduce distillation costs, we have proposed to develop a simultaneous saccharification co-fermentation (SScF) process, where the fermentation of the pentose-containing hydrolysate and cellulosic fraction occurs within the same fermentation vessel. In order to accomplish this goal, improvements in the ethanologens must be made to address a number of issues that arise, including improved hydrolysate tolerance, co-fermentation of the pentose and hexose sugars and increased ethanol tolerance. Using a variety of approaches, including transcriptomics, strain adaptation, metagenomics and directed evolution, this work describes the efforts of a team of scientists from Verenium, University of Florida, Massachusetts Institute of Technology and Genomatica to improve the E. coli and K. oxytoca ethanologens to meet these requirements.
【 预 览 】
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RO201704240000848LZ | 192KB | download |