Role of horizontal gene transfer as a control on the coevolution of ribosomal proteins and the genetic code

Woese, Carl R. ; Goldenfeld, Nigel ; Luthey-Schulten, Zaida

关键词: COMPUTERIZED SIMULATION;    DOCUMENTATION;    GENES;    GENETICS;    PHENOTYPE;    PROTEINS;    RESOLUTION;    RIBOSOMES;    SYNTHESIS Horizontal gene transfer;    aminoacyl-tRNA synthetase;    ribosomal proteins;    models of evolution;    evolutionary analysis software;   

DOI  :  10.2172/1010449
RP-ID  :  DOE ER64144
PID  :  OSTI ID: 1010449
Others  :  Other: A5650
Others  :  TRN: US201209%%495

学科分类：生物科学（综合）

美国|英语

来源: SciTech Connect

【 摘 要 】

Our main goal is to develop the conceptual and computational tools necessary to understand the evolution of the universal processes of translation and replication and to identify events of horizontal gene transfer that occurred within the components. We will attempt to uncover the major evolutionary transitions that accompanied the development of protein synthesis by the ribosome and associated components of the translation apparatus. Our project goes beyond standard genomic approaches to explore homologs that are represented at both the structure and sequence level. Accordingly, use of structural phylogenetic analysis allows us to probe further back into deep evolutionary time than competing approaches, permitting greater resolution of primitive folds and structures. Specifically, our work focuses on the elements of translation, ranging from the emergence of the canonical genetic code to the evolution of specific protein folds, mediated by the predominance of horizontal gene transfer in early life. A unique element of this study is the explicit accounting for the impact of phenotype selection on translation, through a coevolutionary control mechanism. Our work contributes to DOE mission objectives through: (1) sophisticated computer simulation of protein dynamics and evolution, and the further refinement of techniques for structural phylogeny, which complement sequence information, leading to improved annotation of genomic databases; (2) development of evolutionary approaches to exploring cellular function and machinery in an integrated way; and (3) documentation of the phenotype interaction with translation over evolutionary time, reflecting the system response to changing selection pressures through horizontal gene transfer.