Validation of a dynamic evaporation technique for generating isoflurane/air gas mixtures standards to calibrate anesthetic gas monitors

Scientific Reports

卷:16

Article

Aksam Abdelkhalik¹  Ghada Makhlouf¹  Randa N. Yamani² 

[1] Gas Analysis and Fire Safety Laboratory, National Institute of Standards (NIS), P.O. Box 136, El‑Sadat Street, El‑Haram, 12211, El‑Giza, Egypt

[2] Inorganic Analysis and Electrochemical Laboratory, National Institute of Standards (NIS), P.O. Box 136, El‑Sadat Street, El‑Haram, 12211, El‑Giza, Egypt

关键词: Isoflurane–air mixtures;    EN 1839;    Dynamic evaporation;    Calibration gas mixture;    Anesthetic gas monitor;    Method validation;    Measurement uncertainty;   

DOI  :  10.1038/s41598-026-69018-z

 received in 2026-07-13, accepted in 2026-08-25,  发布年份 2026

来源: Springer

【 摘 要 】

The dynamic evaporation and mixing technique standardized in EN 1839 (method T), originally developed to determine the flammability limits of chemical vapors, was applied to prepare isoflurane-in-air calibration gas mixtures, with air serving as the carrier gas. Isoflurane concentrations spanning 0 to 8 vol% were generated on demand by independently controlling the liquid isoflurane and air flow rates. Verification using an anesthetic gas monitor demonstrated excellent agreement between predicted and measured concentrations. A comprehensive validation study was conducted in accordance with EURACHEM guidelines, encompassing linearity, limit of detection (LOD), limit of quantification (LOQ), precision, robustness, and measurement uncertainty. The method yielded a correlation coefficient of r² = 0.9998 over the full clinical range, an LOD of 0.08 vol%, and an LOQ of 0.27 vol%. All concentrations between 0.70 and 8 vol% complied with EN 1839 uncertainty requirements, confirming the suitability of this approach for routine calibration of isoflurane gas monitors.

【 授权许可】

CC BY   
© The Author(s) 2026