Phenotypic and genotypic characterization of clinical Staphylococcus lugdunensis isolates: a French retrospective cohort study

Microbiology Spectrum

卷:14

Research Article

Marin Delaunay¹  François Gravey²  Olivier Join-Lambert²  Yohann Repessé^3,4  Sandrine Dahyot⁵  Martine Pestel-Caron⁵  Jean-Christophe Giard¹  Renaud Verdon⁶  Pascal Thibon⁷  Simon Le Hello²  Christophe Isnard² 

[1] Université de Caen Normandie, Univ Rouen Normandie, Normandie Univ, INSERM, DYNAMICURE UMR 1311, Caen, France

[2] Department of Bacteriology, Univ Rouen Normandie, Université de Caen Normandie, Normandie Univ, INSERM, DYNAMICURE UMR 1311, CHU Rouen, Rouen, France

[3] Department of Biological Hematology, Université de Caen Normandie, INSERM, PhIND UMR1237, GIP Cyceron, CHU Caen Normandie, Caen, France

[4] Department of Infectious Agents, Bacteriology Unit, Université de Caen Normandie, Univ Rouen Normandie, Normandie Univ, INSERM, DYNAMICURE UMR 1311, CHU Caen Normandie, Caen, France

[5] CHU Caen Normandie, Centre d'appui Pour La Prévention Des Infections Associées Aux Soins, CPias Normandie, Caen, France

[6] Department of Tropical Medicine and Infectious Diseases, Université de Caen Normandie, Univ Rouen Normandie, Normandie Univ, INSERM, DYNAMICURE UMR 1311, CHU Caen Normandie, Caen, France

[7] Université de Caen Normandie, CHU Caen Normandie, National Reference Center for Willebrand Disease, Caen, France

关键词: Staphylococcus lugdunensis;    infective endocarditis;    von Willebrand factor;    lugdunin genomic study;    virulence factors;    antibiotic resistance;    prosthetic joint infections;    skin and soft tissue infections;   

DOI  :  10.1128/spectrum.03856-25

 received in 2025-11-28, accepted in 2026-05-28,  发布年份 2026

来源: American Society for Microbiology

【 摘 要 】

ABSTRACTDespite its dominance as a commensal, Staphylococcus lugdunensis (SLU) is a particularly virulent coagulase-negative Staphylococcus that is predominantly associated with community-acquired soft-tissue and skin infections. The aim of our study was to identify genotypic and phenotypic traits associated with the clinical presentations of SLU-associated infections, such as prosthetic joint infections, infective endocarditis (IE), and skin and soft tissue infections. Between January 2001 and December 2016, 73 pathogenic (including 18 strains responsible for IE) and 3 nonpathogenic SLU strains were retrospectively collected from nine French hospitals. All the strains belonged to six clonal complexes (CC1 to CC6) and 12 sequence types (STs). Interestingly, the capacity of the IE-associated strains to bind the von Willebrand factor was greater than that of the other strains, regardless of the CC. Biofilm formation was significantly increased for the most common CCs involved in infections (CC1, CC2, and CC3) and for IE-associated strains. The ability of SLU to inhibit other pathogens varied depending on the CCs caused by specific mutations within the lug operon that resulted in truncated protein expression. Genetic variants from genome virulence genes such as agrC and atIL also vary according to STs. The Galleria mellonella infection model revealed that CC4 was more virulent than the other CCs. On the basis of a large sequenced collection of SLU strains, we found that important phenotypic variation occurred among clinical strains and seemed to be related to some type of infection.IMPORTANCEStaphylococcus lugdunensis is a coagulase-negative Staphylococcus recognized for its virulence in human clinical infections. However, few studies have focused on investigating the phenotypic and genotypic aspects, as well as the population characteristics, of clinical strains involved in severe invasive infections such as endocarditis or skin and soft tissue infections, or of strains associated with so-called cutaneous carriage. Here, we compared different virulence gene variants in terms of populational aspects and also highlighted important phenotypic trait changes according to the clinical presentation and site of isolation of the strains.

【 授权许可】

CC BY   
Copyright © 2026 Delaunay et al.

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