Quantitative analysis of exogenous IGF-1 administration of intervertebral disc through intradiscal injection

JOURNAL OF BIOMECHANICS

卷:45

Article

Huang, C. -Y.²  Gu, W. Y.¹ 

[1] Univ Miami, Dept Mech & Aerosp Engn, Coll Engn, Tissue Biomech Lab, Coral Gables, FL 33124 USA

[2] Univ Miami, Stem Cell & Mechanobiol Lab, Dept Biomed Engn, Coral Gables, FL 33124 USA

关键词: Intervertebral disc;    IGF-1;    Disc degeneration;    Growth factor therapy;   

DOI  :  10.1016/j.jbiomech.2012.02.005

来源: Elsevier

【 摘 要 】

Exogenous administration of IGF-1 has been proposed as a therapy for disc degeneration. The objectives of this study were to develop a numerical model for quantitatively analysing exogenous administration of IGF-1 into the intervertebral disc (IVD) via intradiscal injection and to investigate the effects of IGF-1 administration on distribution of glucose and oxygen in the IVD. In this study, the reversible binding reaction between IGF-1 and IGF binding proteins was incorporated into the mechano-electrochemical mixture model. The model was used to numerically analyse transport of IGF-1, glucose, oxygen and lactate in the IVD after IGF-1 administration. The enhancement of IGF-1 on lactate production was also taken into account in the theoretical model. The numerical analyses using finite element method demonstrated that the binding reactions significantly affect the time-dependent distribution of IGF-1 in the IVD. It was found that the region affected by IGF-1 was smaller and the duration of the therapeutic IGF-1 level was longer in the degenerated disc with a higher concentration of IGF binding proteins. It was also found that the IGF-1 injection can reduce glucose concentration and increase lactate accumulation (i.e., lower pH) in the IVD and these influences were regulated by the IGF-1 binding reactions. This study indicated the complexity of intradiscal administration of growth factors, which needs to be fully analysed in order to achieve a successful outcome. The new theoretical model developed in this study can serve as a powerful tool in analysing and designing the optimal treatments of growth factors for disc degeneration. (C) 2012 Elsevier Ltd. All rights reserved.

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