Elastic modulus and hydraulic permeability of MDCK monolayers

JOURNAL OF BIOMECHANICS

卷:53

Article

Schulze, K. D.¹  Zehnder, S. M.¹  Uruena, J. M.¹  Bhattacharjee, T.¹  Sawyer, W. G.^1,2  Angelini, T. E.^1,3,4 

[1] Univ Florida, Dept Mech & Aerosp Engn, 338 MAE-B, Gainesville, FL 32611 USA

[2] Univ Florida, Dept Mat Sci & Engn, Gainesville, FL 32611 USA

[3] Univ Florida, J Crayton Pruitt Family Dept Biomed Engn, Gainesville, FL USA

[4] Univ Florida, Inst Cell Engn & Regenerat Med, Gainesville, FL USA

关键词: Mesoscale;    Cell mechanics;    Contact mechanics;    Indentation;    Permeability;   

DOI  :  10.1016/j.jbiomech.2017.01.016

来源: Elsevier

【 摘 要 】

The critical role of cell mechanics in tissue health has led to the development of many in vitro methods that measure the elasticity of the cytoskeleton and whole cells, yet the connection between these local cell properties and bulk measurements of tissue mechanics remains unclear. To help bridge this gap, we have developed a monolayer indentation technique for measuring multi-cellular mechanics in vitro. Here, we measure the elasticity of cell monolayers and uncover the role of fluid permeability in these multi-cellular systems, finding that the resistance of fluid transport through cells controls their force response at long times. (C) 2017 Elsevier Ltd. All rights reserved.

【 授权许可】

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