Applied osmotic loading for promoting development of engineered cartilage

JOURNAL OF BIOMECHANICS

卷:46

Article

Sampat, Sonal R.¹  Dermksian, Matthew V.¹  Oungoulian, Sevan R.²  Winchester, Robert J.⁴  Bulinski, J. Chloe³  Ateshian, Gerard A.²  Hung, Clark T.¹ 

[1] Columbia Univ, Dept Biomed Engn, New York, NY 10027 USA

[2] Columbia Univ, Dept Mech Engn, New York, NY 10027 USA

[3] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA

[4] Columbia Univ, Dept Med, New York, NY 10027 USA

关键词: Chondrocytes;    Synovium-derived stem cells;    Tissue engineering;    Cartilage;    Static osmotic loading;   

DOI  :  10.1016/j.jbiomech.2013.07.043

来源: Elsevier

【 摘 要 】

This study investigated the potential use of static osmotic loading as a cartilage tissue engineering strategy for growing clinically relevant grafts from either synovium-derived stem cells (SDSCs) or chondrocytes. Bovine SDSCs and chondrocytes were individually encapsulated in 2% w/v agarose and divided into chondrogenic media of osmolarities 300 (hypotonic), 330 (isotonic), and 400 (hypertonic, physiologic) mOsM for up to 7 weeks. The application of hypertonic media to constructs comprised of SDSCs or chondrocytes led to increased mechanical properties as compared to hypotonic (300 mOsM) or isotonic (330 mOsM) media (p < 0.05). Constant exposure of SDSC-seeded constructs to 400 mOsM media from day 0 to day 49 yielded a Young's modulus of 513 +/- 89 kPa and GAG content of 7.39 +/- 0.52% ww on day 49, well within the range of values of native, immature bovine cartilage. Primary chondrocyte-seeded constructs achieved almost as high a Young's modulus, reaching 487 +/- 187 kPa and 6.77 +/- 0.54%ww (GAG) for the 400 mOsM condition (day 42). These findings suggest hypertonic loading as a straightforward strategy for 3D cultivation with significant benefits for cartilage tissue engineering strategies. In an effort to understand potential mechanisms responsible for the observed response, cell volume measurements in response to varying osmotic conditions were evaluated in relation to the Boyle van't Hoff (BVH) law. Results confirmed that chondrocytes behave as perfect osmometers; however SDSCs deviated from the BVH relation. (C) 2013 Elsevier Ltd. All rights reserved.

【 授权许可】

Free