Biomechanical properties of murine meniscus surface via AFM-based nanoindentation

JOURNAL OF BIOMECHANICS

卷:48

Article

Li, Qing¹  Doyran, Basak¹  Gamer, Laura W.²  Lu, X. Lucas³  Qin, Ling⁴  Ortiz, Christine⁵  Grodzinsky, Alan J.^6,7,8  Rosen, Vicki²  Han, Lin¹ 

[1] Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USA

[2] Harvard Univ, Sch Dent Med, Dept Dev Biol, Boston, MA 02115 USA

[3] Univ Delaware, Dept Mech Engn, Newark, DE 19716 USA

[4] Univ Penn, Dept Orthopaed Surg, Philadelphia, PA 19104 USA

[5] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

[6] MIT, Dept Biol Engn, Cambridge, MA 02139 USA

[7] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA

[8] MIT, Dept Mech Engn, Cambridge, MA 02139 USA

关键词: Meniscus;    Mouse models;    Atomic force microscopy;    Nanoindentation;    Anisotropy;   

DOI  :  10.1016/j.jbiomech.2015.02.064

来源: Elsevier

【 摘 要 】

This study aimed to quantify the biomechanical properties of murine meniscus surface. Atomic force microscopy (AFM)-based nanoindentation was performed on the central region, proximal side of menisci from 6- to 24-week old male C57BL/6 mice using microspherical tips (R-tip approximate to 5 mu m) in PBS. A unique, linear correlation between indentation depth, D, and response force, F, was found on menisci from all age groups. This non-Hertzian behavior is likely due to the dominance of tensile resistance by the collagen fibril bundles on meniscus surface that are mostly aligned along the circumferential direction. The indentation resistance was calculated as both the effective modulus, E-ind, via the isotropic Hertz model, and the effective stiffness, S-ind = dF/dD. Values of S-ind and E-ind were found to depend on indentation rate, suggesting the existence of poro-viscoelasticity. These values do not significantly vary with anatomical sites, lateral versus medial compartments, or mouse age. In addition, E-ind of meniscus surface (e.g., 6.1 +/- 0.8 MPa for 12 weeks of age, mean +/- SEM, n=13) was found to be significantly higher than those of meniscus surfaces in other species, and of murine articular cartilage surface (1.4 +/- 0.1 MPa, n=6). In summary, these results provided the first direct mechanical knowledge of murine knee meniscus tissues. We expect this understanding to serve as a mechanics-based benchmark for further probing the developmental biology and osteoarthritis symptoms of meniscus in various murine models. (C) 2015 Elsevier Ltd. All rights reserved.

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