Tissue-engineered articular cartilage exhibits tension-compression nonlinearity reminiscent of the native cartilage

JOURNAL OF BIOMECHANICS

卷:46

Article

Kelly, Terri-Ann N.¹  Roach, Brendan L.¹  Weidner, Zachary D.⁴  Mackenzie-Smith, Charles R.¹  O'Connell, Grace D.¹  Lima, Eric G.⁵  Stoker, Aaron M.⁶  Cook, James L.⁷  Ateshian, Gerard A.^2,3  Hung, Clark T.¹ 

[1] Columbia Univ, Dept Biomed Engn, Cellular Engn Lab, New York, NY 10027 USA

[2] Columbia Univ, Dept Biomed Engn, Musculoskeletal Biomech Lab, New York, NY USA

[3] Columbia Univ, Dept Mech Engn, New York, NY 10027 USA

[4] St Lukes Roosevelt Hosp, Dept Orthoped Surg, New York, NY 10025 USA

[5] Cooper Union Adv Sci & Art, Dept Mech Engn, Open Source Hardware Lab, New York, NY 10003 USA

[6] Univ Missouri, Coll Vet Med, Comparat Orthopaed Lab, Columbia, MO USA

[7] Univ Missouri, Missouri Orthopaed Inst, Comparat Orthopaed Lab, Columbia, MO USA

关键词: Optimized digital image correlation;    Osmotic loading;    Tensile properties;    Compressive properties;    Collagen;   

DOI  :  10.1016/j.jbiomech.2013.05.017

来源: Elsevier

【 摘 要 】

The tensile modulus of articular cartilage is much larger than its compressive modulus. This tension compression nonlinearity enhances interstitial fluid pressurization and decreases the frictional coefficient. The current set of studies examines the tensile and compressive properties of cylindrical chondrocyte-seeded agarose constructs over different developmental stages through a novel method that combines osmotic loading, video microscopy, and uniaxial unconfined compression testing. This method was previously used to examine tension-compression nonlinearity in native cartilage. Engineered cartilage, cultured under free-swelling (FS) or dynamically loaded (DL) conditions, was tested in unconfined compression in hypertonic and hypotonic salt solutions. The apparent equilibrium modulus decreased with increasing salt concentration, indicating that increasing the bath solution osmolarity shielded the fixed charges within the tissue, shifting the measured moduli along the tension-compression curve and revealing the intrinsic properties of the tissue. With this method, we were able to measure the tensile (401 +/- 83 kPa for FS and 678 +/- 473 kPa for DL) and compressive (161 +/- 33 kPa for FS and 348 +/- 203 kPa for DL) moduli of the same engineered cartilage specimens. These moduli are comparable to values obtained from traditional methods, validating this technique for measuring the tensile and compressive properties of hydrogel-based constructs. This study shows that engineered cartilage exhibits tension-compression nonlinearity reminiscent of the native tissue, and that dynamic deformational loading can yield significantly higher tensile properties. (C) 2013 Elsevier Ltd. All rights reserved.

【 授权许可】

Free