Effect of glutaraldehyde fixation on the frictional response of immature bovine articular cartilage explants

JOURNAL OF BIOMECHANICS

卷:47

Article

Oungoulian, Sevan R.¹  Hehir, Kristin E.¹  Zhu, Kaicen¹  Willis, Callen E.¹  Marinescu, Anca G.¹  Merali, Natasha¹  Ahmad, Christopher S.³  Hung, Clark T.²  Ateshian, Gerard A.^1,2 

[1] Columbia Univ, Dept Mech Engn, New York, NY 10027 USA

[2] Columbia Univ, Dept Biomed Engn, New York, NY 10027 USA

[3] Columbia Univ, Dept Orthopaed Surg, New York, NY 10027 USA

关键词: Cartilage;    Osteoarthritis;    Friction;    Wear;    Damage;   

DOI  :  10.1016/j.jbiomech.2013.11.043

来源: Elsevier

【 摘 要 】

This study examined functional properties and biocompatibility of glutaraldehyde-fixed bovine articular cartilage over several weeks of incubation at body temperature to investigate its potential use as a resurfacing material in joint arthroplasty. In the first experiment, treated cartilage disks were fixed using 0.02, 0.20 and 0.60% glutaraldehyde for 24 h then incubated, along with an untreated control group, in saline for up to 28 d at 37 degrees C. Both the equilibrium compressive and tensile moduli increased nearly twofold in treated samples compared to day 0 control, and remained at that level from day 1 to 28; the equilibrium friction coefficient against glass rose nearly twofold immediately after fixation (day 1) but returned to control values after day 7. Live explants co-cultured with fixed explants showed no quantitative difference in cell viability over 28 d. In general, no significant differences were observed between 0.20 and 0.60% groups, so 0.20% was deemed sufficient for complete fixation. In the second experiment, cartilage-on-cartilage frictional measurements were performed under a migrating contact configuration. In the treated group, one explant was fixed using 0.20% glutaraldehyde while the apposing explant was left untreated; in the control group both explants were left untreated. From day 1 to 28, the treated group exhibited either no significant difference or slightly lower friction coefficient than the untreated group. These results suggest that a properly titrated glutaraldehyde treatment can reproduce the desired functional properties of native articular cartilage and maintain these properties for at least 28 d at body temperature. (C) 2013 Elsevier Ltd. All rights reserved.

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