Peripheral whole blood microRNA alterations in major depression and bipolar disorder

JOURNAL OF AFFECTIVE DISORDERS

卷:200

Article

Maffioletti, Elisabetta¹  Cattaneo, Annamaria^2,3  Rosso, Gianluca⁴  Maina, Giuseppe⁴  Maj, Carlo¹  Gennarelli, Massimo^1,5  Tardito, Daniela⁶  Bocchio-Chiavetto, Luisella^1,7 

[1] IRCCS Ctr S Giovanni Dio, Genet Unit, Brescia, Italy

[2] IRCCS Ctr S Giovanni Dio, Biol Psychiat Unit, Brescia, Italy

[3] Kings Coll London, Dept Psychol Med, Inst Psychiat, London WC2R 2LS, England

[4] Univ Turin, SCDU Psychiat Serv, AOU San Luigi Gonzaga Hosp, Turin, Italy

[5] Univ Brescia, Dept Mol & Translat Med, I-25121 Brescia, Italy

[6] Univ Milan, Dept Pharmacol & Biomol Sci, I-20122 Milan, Italy

[7] ECampus Univ, Fac Psychol, Novedrate, Como, Italy

关键词: MicroRNA;    Major depression;    Bipolar disorder;    Blood;    Biomarker;    Let-7;   

DOI  :  10.1016/j.jad.2016.04.021

来源: Elsevier

【 摘 要 】

Major depression (MD) and bipolar disorder (BD) are severe and potentially life-threating mood disorders whose etiology is to date not completely understood. MicroRNAs (miRNAs) are small non-coding RNAs that regulate protein synthesis post-transcriptionally by base-pairing to target gene mRNAs. Growing evidence indicated that miRNAs might play a key role in the pathogenesis of neuropsychiatric disorders and in the action of psychotropic drugs. On these bases, in this study we evaluated the expression levels of 1733 mature miRNAs annotated in miRBase v.17, through a microarray technique, in the blood of 20 MD and 20 BD patients and 20 healthy controls, in order to identify putative miRNA signatures associated with mood disorders. We found that 5 miRNAs (hsa-let-7a-5p, hsa-let-7d-5p, hsa-let7f-5p, hsa-miR-24-3p and hsa-miR-425-3p) were specifically altered in MD patients and 5 (hsa-miR-140-3p, hsa-miR-30d-5p, hsa-miR-330-5p, hsa-miR-378a-5p and hsa-miR-21-3p) in BD patients, whereas 2 miRNAs (hsa-miR-330-3p and hsa-miR-345-5p) were dysregulated in both the diseases. The bioinformatic prediction of the genes targeted by the altered miRNAs revealed the possible involvement of neural pathways relevant for psychiatric disorders. In conclusion, the observed results indicate a dysregulation of miRNA blood expression in mood disorders and could indicate new avenues for a better understanding of their pathogenetic mechanisms. The identified alterations may represent potential peripheral biomarkers to be complemented with other clinical and biological features for the improvement of diagnostic accuracy. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

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