Tyrosine kinase B protein expression is reduced in the cerebellum of patients with bipolar disorder

JOURNAL OF AFFECTIVE DISORDERS

卷:133

Article

Soontornniyomkij, Benchawanna¹  Everall, Ian P.²  Chana, Gursharan²  Tsuang, Ming T.¹  Achim, Cristian L.¹  Soontornniyomkij, Virawudh¹ 

[1] Univ Calif San Diego, Dept Psychiat, Sch Med, 9500 Gilman Dr, La Jolla, CA 92093 USA

[2] Univ Melbourne, Dept Psychiat, Melbourne, Vic, Australia

关键词: Bipolar disorder;    Brain-derived neurotrophic factor;    Cerebellum;    Major depression;    Schizophrenia;    TrkB;   

DOI  :  10.1016/j.jad.2011.04.044

来源: Elsevier

【 摘 要 】

Background: The role of the cerebellum in coordinating mental activity is supported by its connections with cerebral regions involved in cognitive/affective functioning, with decreased activities on functional neuroimaging observed in the cerebellum of schizophrenia patients performing mental tasks. Brain-derived neurotrophic factor (BDNF)-induced activation of tyrosine kinase B (TrkB) is essential to synaptic plasticity. We hypothesized that alterations in BDNF and TrkB expression in the cerebellum were associated with schizophrenia and affective disorders. Methods: We employed immunohistochemistry and immunoblotting to quantify protein expression of BDNF and TrkB in the cerebellum of patients with schizophrenia, bipolar disorder, and major depression compared to controls (n = 15 each). Results: While TrkB immunoreactivity in each of the molecular and granule-cell layers was reduced in all 3 disease groups (12-34%) compared to the control (P=0.018 and 0.038, respectively, ANOVA), only the reduction in bipolar disorder remained statistically significant upon Tukey-Kramer post hoc analyses (P= 0.019 and 0.021, respectively). Apparent decreases in BDNF immunoreactivity in all 3 disease groups (12-30%) compared to the control were not statistically significant. TrkB immunoreactivity was not significantly associated with any of the demographic, clinical, and postmortem variables. Immunoblotting displayed an 85-kDa TrkB-immunoreactive band, consistent with a truncated isoform, in all 60 cases. Limitations: On immunoblotting. apparent decreases in 85-kDa-TrkB levels in all 3 disease groups compared to the control were not statistically significant. Conclusions: Our finding of reduced TrkB expression in bipolar disorder suggests that dysregulation of TrkB-mediated neurotrophin signaling in the cerebellum may play a role in the pathophysiology of this disease. (C) 2011 Elsevier B.V. All rights reserved.

【 授权许可】

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