Pharmacokinetics, biodistribution and metabolism of squalenoyl adenosine nanoparticles in mice using dual radio-labeling and radio-HPLC analysis

JOURNAL OF CONTROLLED RELEASE

卷:212

Article

Gaudin, Alice¹  Lepetre-Mouelhi, Sinda¹  Mougin, Julie¹  Parrod, Martine²  Pieters, Gregory³  Garcia-Argote, Sebastien³  Loreau, Olivier³  Goncalves, Jordan²  Chacun, Helene¹  Courbebaisse, Yann²  Clayette, Pascal²  Desmaele, Didier¹  Rousseau, Bernard³  Andrieux, Karine¹  Couvreur, Patrick¹ 

[1] Univ Paris 11, Fac Pharm, Inst Galien Paris Sud, UMR CNRS 8612, F-92296 Chatenay Malabry, France

[2] BERTIN Pharma, F-45071 Orleans, France

[3] CEA Saclay, iBiTecS S SCBM, Lab LERMIT, F-91191 Gif Sur Yvette, France

关键词: Nanoparticle;    Biodistribution;   

DOI  :  10.1016/j.jconrel.2015.06.016

来源: Elsevier

【 摘 要 】

Adenosine is a pleiotropic endogenous nucleoside with potential neuroprotective pharmacological activity. However, clinical use of adenosine is hampered by its extremely fast metabolization. To overcome this limitation, we recently developed a new squalenoyl nanomedicine of adenosine [Squalenoyl-Adenosine (SQAd)] by covalent linkage of this nucleoside to the squalene, a natural lipid. The resulting nanoassemblies (NAs) displayed a dramatic pharmacological activity both in cerebral ischemia and spinal cord injury pre-clinical models. The aim of the present study was to investigate the plasma profile and tissue distribution of SQAd NAs using both Squalenoyl-[H-3]-Adenosine NAs and [C-14]-Squalenoyl-Adenosine NAs as respective tracers of adenosine and squalene moieties of the SQAd bioconjugate. This study was completed by radio-HPLC analysis allowing to determine the metabolization profile of SQAd. We report here that SQAd NAs allowed a sustained circulation of adenosine under its prodrug form (SQAd) for at least 1 h after intravenous administration, when free adenosine was metabolized within seconds after injection. Moreover, the squalenoylation of adenosine and its formulation as NAs also significantly modified biodistribution, as SQAd NAs were mainly captured by the liver and spleen, allowing a significant release of adenosine in the liver parenchyma. Altogether, these results suggest that SQAd NAs provided a reservoir of adenosine into the bloodstream which may explain the previously observed neuroprotective efficacy of SQAd NAs against cerebral ischemia and spinal cord injury. (C) 2015 The Authors. Published by Elsevier B.V.

【 授权许可】

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