A corrole nanobiologic elicits tissue-activated MRI contrast enhancement and tumor-targeted toxicity

JOURNAL OF CONTROLLED RELEASE

卷:217

Article

Sims, Jessica D.¹  Hwang, Jae Youn⁴  Wagner, Shawn^1,2  Alonso-Valenteen, Felix¹  Hanson, Chris¹  Taguiam, Jan Michael¹  Polo, Richard¹  Harutyunyan, Ira⁵  Karapetyan, Gevorg⁵  Sorasaenee, Karn⁵  Ibrahim, Ahmed^1,3  Marban, Eduardo³  Moats, Rex⁵  Gray, Harry B.⁶  Gross, Zeev⁷  Medina-Kauwe, Lali K.^1,8 

[1] Cedars Sinai Med Ctr, Dept Biomed Sci, Los Angeles, CA 90048 USA

[2] Cedars Sinai Med Ctr, Biomed Imaging Res Inst, Los Angeles, CA 90048 USA

[3] Cedars Sinai Med Ctr, Inst Heart, Los Angeles, CA 90048 USA

[4] Daegu Gyeongbuk Inst Sci & Technol, Daegu, South Korea

[5] USC, Keck Sch Med, Childrens Hosp, Translat Biomed Imaging Lab,Dept Radiol,Saban Res, Los Angeles, CA USA

[6] CALTECH, Dept Chem, Pasadena, CA 91125 USA

[7] Technion Israel Inst Technol, Schulich Fac Chem, Haifa, Israel

[8] Univ Calif Los Angeles, Geffen Sch Med, Los Angeles, CA 90024 USA

关键词: Nanoparticle;    Tumor-targeting;    Corrole;    Manganese;    MRI;   

DOI  :  10.1016/j.jconrel.2015.08.046

来源: Elsevier

【 摘 要 】

Water-soluble corroles with inherent fluorescence can form stable self-assemblies with tumor-targeted cell penetration proteins, and have been explored as agents for optical imaging and photosensitization of tumors in pre-clinical studies. However, the limited tissue-depth of excitation wavelengths limits their clinical applicability. To examine their utility in more clinically-relevant imaging and therapeutic modalities, here we have explored the use of corroles as contrast enhancing agents for magnetic resonance imaging (MRI), and evaluated their potential for tumor-selective delivery when encapsulated by a tumor-targeted polypeptide. We have found that a manganese-metallated corrole exhibits significant T1 relaxation shortening and MRI contrast enhancement that is blocked by particle formation in solution but yields considerable MRI contrast after tissue uptake. Cell entry but not low pH enables this. Additionally, the corrole elicited tumor-toxicity through the loss of mitochondrial membrane potential and cytoskeletal breakdown when delivered by the targeted polypeptide. The protein-corrole particle (which we call HerMn) exhibited improved therapeutic efficacy compared to current targeted therapies used in the clinic. Taken together with its tumor-preferential biodistribution, our findings indicate that HerMn can facilitate tumor-targeted toxicity after systemic delivery and tumor-selective MR imaging activatable by internalization. (c) 2015 Elsevier B.V. All rights reserved.

【 授权许可】

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