Sustained reduction of intraocular pressure by supraciliary delivery of brimonidine-loaded poly(lactic acid) microspheres for the treatment of glaucoma

JOURNAL OF CONTROLLED RELEASE

卷:228

Article

Chiang, B.¹  Kim, Y. C.²  Doty, A. C.^3,5  Grossniklaus, H. E.⁴  Schwendeman, S. P.³  Prausnitz, M. R.^1,2 

[1] Georgia Inst Technol, Wallace H Coulter Dept Biomed Engn, Atlanta, GA 30332 USA

[2] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA

[3] Univ Michigan, Biointerfaces Inst, Dept Pharmaceut Sci, North Campus Res Complex,2800 Plymouth Rd, Ann Arbor, MI 48109 USA

[4] Emory Univ, Sch Med, Dept Ophthalmol, Atlanta, GA 30322 USA

[5] Merck Sharp & Dohme Corp, Pharmaceut Sci & Clin Supply, Discovery Pharmaceut Sci, 33 Ave Louis Pasteur, Boston, MA 02460 USA

关键词: Suprachoroidal space;    Glaucoma;    Brimonidine;    Microneedles;    New Zealand White rabbit;   

DOI  :  10.1016/j.jconrel.2016.02.041

来源: Elsevier

【 摘 要 】

Although effective drugs that lower intraocular pressure (IOP) in the management of glaucoma exist, their efficacy is limited by poor patient adherence to the prescribed eye drop regimen. To replace the need for eye drops, in this study we tested the hypothesis that IOP can be reduced for one month after a single targeted injection using a microneedle for administration of a glaucoma medication (i.e., brimonidine) formulated for sustained release in the supraciliary space of the eye adjacent to the drug's site of action at the ciliary body. To test this hypothesis, brimonidine-loaded microspheres were formulated using poly(lactic acid) (PLA) to release brimonidine at a constant rate for 35 days and microneedles were designed to penetrate through the sclera, without penetrating into the choroid/retina, in order to target injection into the supraciliary space. A single administration of these microspheres using a hollow microneedle was performed in the eye of New Zealand White rabbits and was found to reduce IOP initially by 6 mm Hg and then by progressively smaller amounts for more than one month. All administrations were well tolerated without significant adverse events, although histological examination showed a foreign-body reaction to the microspheres. This study demonstrates, for the first time, that the highly-targeted delivery of brimonidine-loaded microspheres into the supraciliary space using a microneedle is able to reduce IOP for one month as an alternative to daily eye drops. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

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