Enhanced magnetic resonance imaging of experimental pancreatic tumor in vivo by block copolymer-coated magnetite nanoparticles with TGF-β inhibitor

JOURNAL OF CONTROLLED RELEASE

卷:140

Article; Proceedings Paper

Kumagai, Michiaki^1,5  Kano, Mitsunobu R.^2,6  Morishita, Yasuyuki²  Ota, Motomi¹  Imai, Yutaka¹  Nishiyama, Nobuhiro^1,5,6  Sekino, Masaki³  Ueno, Shoogo⁴  Miyazono, Kohei^2,6  Kataoka, Kazunori^1,5,6 

[1] Univ Tokyo, Grad Sch Engn, Dept Mat Engn, Bunkyo Ku, Tokyo 1138656, Japan

[2] Univ Tokyo, Grad Sch Med, Dept Mol Pathol, Bunkyo Ku, Tokyo 1130033, Japan

[3] Univ Tokyo, Grad Sch Frontier Sci, Dept Adv Energy, Kashiwa, Chiba 2778561, Japan

[4] Kyushu Univ, Grad Sch Engn, Dept Appl Quantum Phys, Higashi Ku, Fukuoka 8128581, Japan

[5] Univ Tokyo, Sch Med, Ctr Dis Biol & Integrat Med, Bunkyo Ku, Tokyo 1130033, Japan

[6] Univ Tokyo, Ctr NanoBio Integrat, Bunkyo Ku, Tokyo 1138656, Japan

关键词: Magnetic resonance imaging;    Pancreatic cancer;    TGF-beta;    Magnetite nanoparticles;    Poly(ethylene glycol);   

DOI  :  10.1016/j.jconrel.2009.06.002

来源: Elsevier

【 摘 要 】

Early detection of solid tumors, particularly pancreatic cancer, is of substantial importance in clinics. Enhanced magnetic resonance imaging (MRI) with iron oxide nanoparticles is an available way to detect the cancer. The effective and selective accumulation of these nanoparticles in the tumor tissue is needed for improved imaging, and in this regard, their longevity in the blood circulation time is crucial. We developed here block copolymer-coated magnetite nanoparticles for pancreatic cancer imaging, by means of a chelation between the carboxylic acid groups in poly(ethylene glycol)-poly(aspartic acid) block copolymer (PEG-PAsp) and Fe on the surface of the iron oxide nanoparticles. These nanoparticles had considerably narrow distribution, even upon increased ionic strength or in the presence of fetal bovine serum. The PEG-PAsp-coated nanoparticles were further shown to be potent as a contrast agent for enhanced MRI for an experimental pancreatic cancer, xenografts of the human-derived BxPC3 cell line in BALB/c nude mice, with combined administration of TGF-beta inhibitor. Iron staining of tumor tissue confirmed the accumulation of the nanoparticles in tumor tissue. Use of the PEG-PAsp-coated magnetite nanoparticles, combined with the TGF-beta inhibitor, is of promising clinical importance for the detection of intractable solid cancers, including pancreatic cancer. (C) 2009 Elsevier B.V. All rights reserved.

【 授权许可】

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