Multiparameter evaluation of in vivo gene delivery using ultrasound-guided, microbubble-enhanced sonoporation

JOURNAL OF CONTROLLED RELEASE

卷:223

Article

Shapiro, Galina¹  Wong, Andrew W.²  Bez, Maxim¹  Yang, Fang²  Tam, Sarah²  Even, Lisa²  Sheyn, Dmitriy^3,4  Ben-David, Shiran^3,4  Tawackoli, Wafa^3,4  Pelled, Gadi^3,4  Ferrara, Katherine W.²  Gazit, Dan^1,3,4,5 

[1] Hebrew Univ Jerusalem, Hadassah Fac Dent Med, Skeletal Biotech Lab, IL-91120 Jerusalem, Israel

[2] Univ Calif Davis, Dept Biomed Engn, 451 Hlth Sci Dr, Davis, CA 95616 USA

[3] Cedars Sinai Med Ctr, Dept Surg, 8700 Beverly Blvd, Los Angeles, CA 90048 USA

[4] Cedars Sinai Med Ctr, Board Governors Regenerat Med Inst, Los Angeles, CA 90048 USA

[5] Cedars Sinai Med Ctr, Biomed Imaging Res Inst, Los Angeles, CA 90048 USA

关键词: Ultrasound;    Topic;    Microbubbles;    Sonoporation;    Gene therapy;   

DOI  :  10.1016/j.jconrel.2015.12.001

来源: Elsevier

【 摘 要 】

More than 1800 gene therapy clinical trials worldwide have targeted a wide range of conditions including cancer, cardiovascular diseases, and monogenic diseases. Biological (i.e. viral), chemical, and physical approaches have been developed to deliver nucleic acids into cells. Although viral vectors offer the greatest efficiency, they also raise major safety concerns including carcinogenesis and immunogenicity. The goal of microbubble-mediated sonoporation is to enhance the uptake of drugs and nucleic acids. Insonation of microbubbles is thought to facilitate two mechanisms for enhanced uptake: first, deflection of the cell membrane inducing endocytotic uptake, and second, microbubble jetting inducing the formation of pores in the cell membrane. We hypothesized that ultrasound could be used to guide local microbubble-enhanced sonoporation of plasmid DNA. With the aim of optimizing delivery efficiency, we used nonlinear ultrasound and bioluminescence imaging to optimize the acoustic pressure, microbubble concentration, treatment duration, DNA dosage, and number of treatments required for in vivo Luciferase gene expression in a mouse thigh muscle model. We found that mice injected with 50 mu g luciferase plasmid DNA and 5 x 10(5) microbubbles followed by ultrasound treatment at 1.4 MHz, 200 kPa, 100-cycle pulse length, and 540 Hz pulse repetition frequency (PRF) for 2 min exhibited superior transgene expression compared to all other treatment groups. The bioluminescent signal measured for these mice on Day 4 post-treatment was 100-fold higher (p < 0.0001, n=5 or 6) than the signals for controls treated with DNA injection alone, DNA and microbubble injection, or DNA injection and ultrasound treatment. Our results indicate that these conditions result in efficient gene delivery and prolonged gene expression (up to 21 days) with no evidence of tissue damage or off-target delivery. We believe that these promising results bear great promise for the development of microbubble-enhanced sonoporation-induced gene therapies. (C) 2015 Elsevier B.V. All rights reserved.

【 授权许可】

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