Spray-drying of inhalable, multifunctional formulations for the treatment of biofilms formed in cystic fibrosis

JOURNAL OF CONTROLLED RELEASE

卷:314

Article

Lababidi, Nashrawan¹  Kissi, Eric Ofosu^2,3  Elgaher, Walid A. M.⁴  Sigal, Valentin¹  Haupenthal, Joerg⁴  Schwarz, Bianca C.⁵  Hirsch, Anna K. H.^4,6  Rades, Thomas³  Schneider, Marc¹ 

[1] Saarland Univ, Dept Pharm Biopharmaceut & Pharmaceut Technol, Campus C4 1, D-66123 Saarbrucken, Germany

[2] Univ Oslo, Fac Math & Nat Sci, Dept Pharm, N-0316 Oslo, Norway

[3] Univ Copenhagen, Fac Hlth & Med Sci, Dept Pharm, Univ Parken 2, DK-2100 Copenhagen, Denmark

[4] Helmholtz Ctr Infect Res HZI, Helmholtz Inst Pharmaceut Res Saarland HIPS, Dept Drug Design & Optimizat, Campus E8 1, D-66123 Saarbrucken, Germany

[5] Pferdeklin Altforweiler, Raiffeisenstr 100, D-66802 Uberherrn, Germany

[6] Saarland Univ, Med Chem, Dept Pharm, Campus E8 1, D-66123 Saarbrucken, Germany

关键词: Salt formation;    Antibiotics;    Co-amorphous drug delivery;    Inhalation;    Bacterial infection;    Dry-powder formulation;   

DOI  :  10.1016/j.jconrel.2019.10.038

来源: Elsevier

【 摘 要 】

Cystic fibrosis (CF) is a serious lung disease, commonly susceptible to Pseudomonas aeruginosa colonization. The dense mucus together with biofilm formation limit drug permeability and prevent the drug from reaching the site of action, causing treatment failure of the bacterial infection. Besides the use of antibiotics, the mucolytic agent N-acetylcysteine (NAC) is recommended to be co-administered in the treatment of CF. Although several formulations have been developed for inhalation therapy to improve the pulmonary condition in CF patients, there is still no comprehensive study on a combined multifunctional dry powder formulation of antibiotics with NAC. In this work, we developed an innovative multifunctional dry powder inhaler (DPI) formulation based on salt formation between NAC and antibiotics and characterized their solid state properties and physical stability. NAC could be spray dried together with three different antibiotics, azithromycin (Azi), tobramycin (Tobra) and ciprofloxacin (Cipro), without the use of organic solvents to form Azi/NAC, Tobra/NAC and Cipro/NAC DPI formulations. Solid-state characterization of these DPI formulations showed that they were amorphous after spray drying. Azi/NAC and Tobra/NAC form co-amorphous salt systems that were physically stable under storage at stress conditions. For particle characterization, the obtained mass median aerodynamic diameters were in a suitable range for inhalation (< 5.0 mu m). The multifunctional antibiotic/NAC formulations conserved or improved the antibiotic susceptibility and showed promising results regarding the inhibition of P. aeruginosa PA14 biofilm formation.

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