Hybrid polymeric hydrogels via peptide nucleic acid (PNA)/DNA complexation

JOURNAL OF CONTROLLED RELEASE

卷:220

Article; Proceedings Paper

Chu, Te-Wei¹  Feng, Jiayue²  Yang, Jiyuan¹  Kopecek, Jindrich^1,3 

[1] Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA

[2] Univ Utah, Dept Chem, Salt Lake City, UT 84112 USA

[3] Univ Utah, Dept Bioengn, Salt Lake City, UT 84112 USA

关键词: Hydrogel;    Self-assembly;    Peptide nucleic acid;    HPMA polymer;    DNA;    P-form;   

DOI  :  10.1016/j.jconrel.2015.09.035

来源: Elsevier

【 摘 要 】

This work presents a new concept in hybrid hydrogel design. Synthetic water-soluble N-(2-hydroxypropyl) methacrylamide (HPMA) polymers grafted with multiple peptide nucleic acids (PNAs) are crosslinked upon addition of the linker DNA. The self-assembly is mediated by the PNA-DNA complexation, which results in the formation of hydrophilic polymer networks. We show that the hydrogels can be produced through two different types of complexations. Type I hydrogel is formed via the PNA/DNA double-helix hybridization. Type II hydrogel utilizes a unique P-form oligonucleotide triple-helix that comprises two PNA sequences and one DNA. Microrheology studies confirm the respective gelation processes and disclose a higher critical gelation concentration for the type I gel when compared to the type II design. Scanning electron microscopy reveals the interconnected microporous structure of both types of hydrogels. Type I double-helix hydrogel exhibits larger pore sizes than type II triple-helix gel. The latter apparently contains denser structure and displays greater elasticity as well. The designed hybrid hydrogels have potential as novel biomaterials for pharmaceutical and biomedical applications. (C) 2015 Elsevier B.V. All rights reserved.

【 授权许可】

Free