Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation

JOURNAL OF CONTROLLED RELEASE

卷:112

Article

Ito, Tomoko ; Iida-Tanaka, Naoko ; Niidome, Takuro ; Kawano, Takahito ; Kubo, Koji ; Yoshikawa, Kenichi ; Sato, Toshinori ; Yang, Zhihong ; Koyama, Yoshiyuki

关键词: transfection;    hyaluronic acid;    transcription;    HMG;   

DOI  :  10.1016/j.jconrel.2006.03.013

来源: Elsevier

【 摘 要 】

Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasrnid/PEI complexes in CHO cells. (c) 2006 Elsevier B.V All rights reserved.

【 授权许可】

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