Kinetics of functionalised carbon nanotube distribution in mouse brain after systemic injection: Spatial to ultra-structural analyses

JOURNAL OF CONTROLLED RELEASE

卷:224

Article

Wang, Julie T. -W.¹  Rubio, Noelia¹  Kafa, Houmam¹  Venturelli, Enrica²  Fabbro, Chiara³  Menard-Moyon, Cecilia²  Da Ros, Tatiana³  Sosabowski, Jane K.⁴  Lawson, Alastair D.⁵  Robinson, Martyn K.⁵  Prato, Maurizio³  Bianco, Alberto²  Festy, Frederic⁶  Preston, Jane E.¹  Kostarelos, Kostas⁷  Al-Jamal, Khuloud T.¹ 

[1] Kings Coll London, Fac Life Sci & Med, Inst Pharmaceut Sci, London SE1 9NH, England

[2] CNRS, Inst Biol Mol & Cellulaire, Lab Immunopathol & Chim Therapeut, F-67000 Strasbourg, France

[3] Univ Trieste, Dipartimento Sci Chim & Farmaceut, I-34127 Trieste, Italy

[4] Queen Mary Univ London, Barts Canc Inst, Ctr Mol Oncol, London EC1M 6BQ, England

[5] UCB Celltech, Slough SL1 3WE, Berks, England

[6] Kings Coll London, Tissue Engn & Biophoton, Inst Dent, London SE1 9RT, England

[7] UCL, Nanomed Lab, UCL Sch Pharm, Brunswick Sq, London, England

关键词: Carbon nanotubes;    Brain drug delivery;    Blood-brain barrier;    Nanomedicine;    SPECT/CT imaging;    Multi-photon luminescence microscopy;   

DOI  :  10.1016/j.jconrel.2015.12.039

来源: Elsevier

【 摘 要 】

Earlier studies proved the success of using chemically functionalised multi-walled carbon nanotubes (f-MWNTs) as nanocarriers to the brain. Little insight into the kinetics of brain distribution of f-MWNTs in vivo has been reported. This study employed a wide range of qualitative and quantitative techniques with the aim of shedding the light on f-MWNT's brain distribution following intravenous injection. gamma-Scintigraphy quantified the uptake of studied radiolabelled f-MWNT in the whole brain parenchyma and capillaries while 3D-single photon emission computed tomography/computed tomography imaging and autoradiography illustrated spatial distribution within various brain regions. Raman and multiphoton luminescence together with transmission electron microscopy confirmed the presence of intact f-MWNT in mouse brain, in a label-free manner. The results evidenced the presence of f-MWNT in mice brain parenchyma, in addition to brain endothelium. Such information on the rate and extent of regional and cellular brain distribution is needed before further implementation into neurological therapeutics can be made. (C) 2016 The Authors. Published by Elsevier B.V.

【 授权许可】

Free