Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture

JOURNAL OF CONTROLLED RELEASE

卷:164

Article; Proceedings Paper

Bhattacharya, Madhushree^1,2  Malinen, Melina M.^1,2  Lauren, Patrick¹  Lou, Yan-Ru¹  Kuisma, Saara W.¹  Kanninen, Liisa¹  Lille, Martina³  Corlu, Anne⁴  GuGuen-Guillouzo, Christiane⁴  Ikkala, Olli⁵  Laukkanen, Antti⁶  Urtti, Arto²  Yliperttula, Marjo¹ 

[1] Univ Helsinki, Fac Pharm, Div Biopharmaceut & Pharmacokinet, FI-00014 Helsinki, Finland

[2] Univ Helsinki, Fac Pharm, Ctr Drug Res, FI-00014 Helsinki, Finland

[3] VTT Tech Res Ctr Finland, FI-02044 Espoo, Finland

[4] Univ Rennes 1, INSERM, UMR991, F-35043 Rennes, France

[5] Aalto Univ Helsinki Univ Technol, Dept Appl Phys, FI-00076 Espoo, Finland

[6] UPM Kymmene Corp, FI-02150 Espoo, Finland

关键词: 3D cell culture;    Hepatocyte;    Nanocellulose;    Nanofiber;    Plant-derived cellulose;   

DOI  :  10.1016/j.jconrel.2012.06.039

来源: Elsevier

【 摘 要 】

Over the recent years, various materials have been introduced as potential 3D cell culture scaffolds. These include protein extracts, peptide amphiphiles, and synthetic polymers. Hydrogel scaffolds without human or animal borne components or added bioactive components are preferred from the immunological point of view. Here we demonstrate that native nanofibrillar cellulose (NFC) hydrogels derived from the abundant plant sources provide the desired functionalities. We show 1) rheological properties that allow formation of a 3D scaffold in-situ after facile injection, 2) cellular biocompatibility without added growth factors, 3) cellular polarization, and 4) differentiation of human hepatic cell lines HepaRG and HepG2. At high shear stress, the aqueous NFC has small viscosity that supports injectability, whereas at low shear stress conditions the material is converted to an elastic gel. Due to the inherent biocompatibility without any additives, we conclude that NFC generates a feasible and sustained microenvironment for 3D cell culture for potential applications, such as drug and chemical testing, tissue engineering, and cell therapy. (c) 2012 Elsevier B.V. All rights reserved.

【 授权许可】

Free