Transferrin-bearing polypropylenimine dendrimer for targeted gene delivery to the brain

JOURNAL OF CONTROLLED RELEASE

卷:188

Article

Somani, Sukrut¹  Blatchford, David R.¹  Millington, Owain¹  Stevenson, M. Lynn²  Dufes, Christine¹ 

[1] Univ Strathclyde, Strathclyde Inst Pharm & Biomed Sci, Glasgow G4 0RE, Lanark, Scotland

[2] Univ Glasgow, Sch Vet Med, Glasgow G61 1QH, Lanark, Scotland

关键词: Brain delivery;    Blood-brain barrier;    Gene delivery;    Dendrimer;    Transferrin;   

DOI  :  10.1016/j.jconrel.2014.06.006

来源: Elsevier

【 摘 要 】

The possibility of using genes as medicines to treat brain diseases is currently limited by the lack of safe and efficacious delivery systems able to cross the blood-brain barrier, thus resulting in a failure to reach the brain after intravenous administration. On the basis that iron can effectively reach the brain by using transferrin receptors for crossing the blood-brain barrier, we propose to investigate if a transferrin-bearing generation 3-polypropylenimine dendrimer would allow the transport of plasmid DNA to the brain after intravenous administration. In vitro, the conjugation of transferrin to the polypropylenimine dendrimer increased the DNA uptake by bEnd.3 murine brain endothelioma cells overexpressing transferrin receptors, by about 1.4-fold and 23-fold compared to that observed with the non-targeted dendriplex and naked DNA. This DNA uptake appeared to be optimal following 2 h incubation with the treatment. In vivo, the intravenous injection of transferrin-bearing dendriplex more than doubled the gene expression in the brain compared to the unmodified dendriplex, while decreasing the non-specific gene expression in the lung. Gene expression was at least 3-fold higher in the brain than in any tested peripheral organs and was at its highest 24 h following the injection of the treatments. These results suggest that transferrin-bearing polypropylenimine dendrimer is a highly promising gene delivery system to the brain. (C) 2014 Elsevier B.V. All rights reserved.

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